Abstract: A study of the adsorption density and spectra distribution of adsorbed lysozyme (protein) from aqueous solutions unto silica and polystyrene surfaces has been carried out as a function of pH and temperature respectively. The maximum adsorption of lysozyme on both surfaces occurred at the pH range of 10.0 to 12.0 with a peaked value at 11.0. This behaviour showed that lysozyme (protein) has maximum adsorption around its isoelectric point of pH 11.0. The spectra of the adsorbed lysozyme were observed for varying temperature values on the two surfaces. The spectra showed maximum absorbance of 0.8020 at 20°C for silica and 0.7860 at 50°C for polystyrene at the same wavelength of 500 nm. The minimum absorbance occurred at the same wavelength of 600 nm and temperature of 40°C corresponds to the minimum absorbance of 0.004 for silica and 0.028 for polystyrene. Comparatively, minimum absorbance occurred at the same wavelength and temperature for both surfaces, but in contrast, maximum absorbance, though occurred at the same wavelength, but at different temperature; a phenomenon attributed to the non-synthetic nature of silica and synthetic nature of polystyrene surfaces.