Abstract: The chemistry of the Ikogosi Warm Spring in South West Nigeria has been studied. Four out of the six warm spring primary sources show close similarity in temperature, pH, dissolved solids, suspended solid, alkalinity, hardness, SO42-, Cl- and trace metals. All measured values are within classification as fresh water and WHO standards. Further study of the warm spring flow reveal a time (distance) dependent atmospheric environmental induced variations. These variations were made manifest through the optimization of AAS machine parameters such as observation height, PMT voltage, hollow cathode lamp current and optical beam wavelength. A comparison has also been made between the warm spring, a cold stream and the normal ground water of the area through direct and simultaneous measurement of the hiked parameters. Major differences in T, hardness, SO42-, Ca, Mg, Na, K, Fe, Zn, Pb and Cd contents imply the existence of unexplored processes on the spring water.