Abstract: The field experiment was conducted to determine the effect of irrigation frequencies applied at different growth stages on the newly evolved wheat genotypes at Agronomy Experimental Field, Agriculture Research Institute, Tando Jam during winter, 1998-99. Three irrigation frequencies 5, 4 and 3 with four wheat genotypes (Mehran-89, Abadgar-93, 7001 and 7002) were included in the study. Plant height, tillers, earheads, seeds per spike, seed weight, seed index, grain yield varied significantly by the irrigation frequencies and genotypes, and their interaction. Application of 5 irrigation resulated in higher grain yield. Among the four varieties tested newly devloped genotypes 7001 and 7002 yielded more than existing (Mehran-89 and Abadgar-93) genotypes.