Abstract: Background and Objective: Millions of hectares of acid sulphate soils in Indonesia have been cultivated with low yield. The study was carried out to analyze the rate of durian wood waste biochar to improve soil properties and rice yield on acid sulphate soil. Materials and Methods: The research was performed from September, 2017-July, 2018. Biochar was made by pyrolysis of feedstock at 550°C for 2 h and quenched with water. The treatments were biochar rates of 4, 8, 12, 16 and 20 t ha–1 which added on the acid sulphate soil. Analyses of soil characteristics were made at the end of vegetative, generative and harvest phases. This study was used completely randomized design (CRD) with three replications. Results: Along with increasing biochar rate and planting periods, the properties of acid sulphate soils were significantly increased, except for exchangeable Mg. There was no significant difference in plant growth with different rate of biochars, meanwhile, increasing biochar rate significantly increased the filled grain weight of rice. Conclusion: At the end of vegetative, generative and harvest phases, biochar rate had positive effects on pH-H2O, SOC, available P, total N, total K, exchangeable bases of Ca, K, Na and CEC. Durian wood biochar provided the optimum weight of filled grain at a rate of 15.29 t ha–1 and maximum plant height of 16.47 t ha–1 in the acid sulphate soil.