Abstract: The field experiments were conducted during 2004 and 2005 seasons to study the effect of three first post planting irrigation times and ten fertilization treatments on growth, yield, yield components and fiber traits in cotton. The times of first post planting irrigation in a separate experiment were 20, 30 and 40 days from planting irrigation time in both seasons 2004 and 2005. A completely randomized block design with four replicate was used for each time of first irrigation. Each experiment of the first irrigation included ten of fertilizers treatments. Application the first irrigation after 40 days from planting resulted in the highest values of all growth characters. However, application of the first irrigation after 30 days from planting gave the highest values of all yield and yield components, except boll weight which recorded highest values with application the first irrigation after 20 days from planting. On the other hand, the lowest values of all growth, yield and yield components were recorded by application of the first irrigation after 20 days from planting in the two seasons, except boll weight which recorded the lowest values with application the first irrigation after 30 days from planting in the two seasons. Application the first irrigation after 40 days from planting gave the highest values of fiber strength and fiber length uniformity ratio (%). On the other hand, the lowest values of fiber strength and fiber uniformity ratio (%) were recorded by application the first irrigation after 20 days from planting over the two seasons. Maximum means of all studied characters were produced from fertilizing cotton plants with organic fertilizer + bio-fertilizer + 66% from recommended NPK in both seasons. On the contrary, the lowest values were obtained from plots received Bio-fertilizer alone, except fiber quality which the lowest values were obtained from plots received organic fertilizer alone. It can be concluded that applying first irrigation after 30 days from planting date and applying to soil bio-fertilizer (EM) at rate 5 L/fed., organic-fertilizer at rate 20 kg N/fed., in addition to application of 2/3 NPK from recommended may be maximize the productivity of cotton.