Abstract: Four lignocellulosic substrates (sawdust, peat of coconut husk, narrow leaf cattails and bagasse) were used for Pleurotus ostreatus cultivation and 3-6 flushes were obtained from these substrates. A bagasse substrate accelerated the mushroom growing processes. The mycelial completed colonization, primordium initiation and fruiting body formation were found within 28, 40 and 44 days, respectively. The sawdust (control) gave the maximum mushroom yield (536.85 g per 1 kg substrate) and this yield was significantly different to those found from bagasse (360.84 g), peat of coconut husk (278.78 g) and narrow leaf cattails (112.10 g) at a confidence level of 95%. Even the mushroom yield obtained from bagasse was lower than sawdust but in term of biological efficiency (BE) value the result achieved from bagasse (103.56%) was slightly higher than sawdust (95.02%). Low BE values were revealed in both peat of coconut husk (56.76%) and narrow leaf cattails (44.67%) and these values were significantly different and two times less than those found in bagasse and sawdust. Therefore, when BE value was taking into account the lignocellulosic substrate likes bagasse has shown great potential for use as a raw material instead of sawdust since this substrate provides an economically acceptable production alternative for P. ostreatus cultivation.