Abstract: Early and late cold stress in fall and spring, saline, drought and heat stress are also some important factors for decreasing percent and rate of germination in wheat. Osmotic priming increases the rate of germination and monotonousness of emergence and decrease undesirable effects of stress by changing on activity of some seed enzymes. In present study, we investigated effects of osmotic priming treatments in different osmotic potential and period of priming, in two temperatures (10 and 20°C) and two local seed masses of Sardary wheat (Triticum aestivum) cultivar. Experiment was planned on base of Randomized complete block design in form of factorial with three replications and three factors in seed laboratory of agricultural collage of Tehran University. Some germination characters such as rate of germination, length of radicle and caulicle, dry weight of seedling and vigor were measured. Results showed the significant effect of temperature and osmotic priming treatments in addition, interactions between them for measured characters were significant. Results of comparing means showed that osmotic priming treatments had no positive significant effects on germination characters, it is possible because of high osmotic potential and long priming period that cause prevention of water absorption and produce free radical of oxygen that destroys the cell membrane. In this situation LEA (late embryogenesis abundant) protein was also degraded so all of this cases cause decrease of the rate of germination.