Abstract: Real time quality control based on machine visual in sheet material industries is becoming a crucial technology. During the defect inspection process, surface image acquisition which is the first significant step, is challenging since fast manufacture speed and various tough condition. Choosing DS90CR288A, DS90LV047 and DS90LV019 for series-parallel data conversion and command interaction, this study presents a hardware architectural structure and turns it into reality, then develops driver program embedded into Field Programmable Gate Array (FPGA), we reduces the signal crosstalk through using reasonable termination, differential pairs controlling and the division of ground etc. The data transfer rate of this image acquisition system can reach up to 5.12 Gbps when pixel clock is up to 80 MHZ with an excellent performance. And it has already been applied to a hot rolling strip surface inspection system steadily for more than one year.