Abstract: This study treated the hospitals as subjects and probed into the correlation among market orientation, organizational learning, types of operational strategies, organizational culture, executive degree of knowledge management activities and operational performance. The samples were 466 qualified hospitals in hospital evaluation and teaching hospital evaluation from 2007 to 2011 registered in Department of Health, Taiwan. The researcher collected related data by distributing questionnaires through mails. The subjects were the supervisors in the hospitals. The results found that the higher executive degree of knowledge management was, the more significant and positive effect it would be on operational performance; the higher the executive degrees of cost leadership, marketing differentiation and innovation differentiation strategy were, the more significant and positive effect it would be on executive degree of knowledge management; the higher the executive degrees of organizational learning and market orientation were, the more significant and positive effect it would be on executive degree of knowledge management; the higher the executive degrees of rational culture, hierarchical culture, group culture and developmental culture were, the more the effect it would be on executive degree of knowledge management. Thus, this study suggests that hospitals should enhance organizational learning, market orientation and knowledge management execution to improve operational performance. Hospital management should value rational culture, hierarchical culture, group culture, developmental culture and initiate cost leadership and differentiation implementation strategies.