Abstract: Our attentiveness and concentration are pilfered from us by the proceedings take place around us in the world in recent times. Different challenges and impediments are faced by the people work in the industries, offices and even in business. It is tough to handle the stress some times. Therefore, to come out of the aforementioned troubles, meditation is essential for all human beings. In the same way, for pschychological stress, speech signal is uttered to be a considerable indicator. In the direction of mediating human subject, OM is a spiritual mantra, outstanding to fetch peace and calm. The entire psychological pressure and worldly thoughts are taken away by the chanting of OM mantra. Elimination of disruption and introduction of new dynamism in the body are given by the OM chanting. The consciousness could be promoted through the repetition of OM mantra. Furthermore, this mantra transcends the restrictions of a mentality. To systematically understand the meditative chant, termed the divine sound OM, is the endeavor of this research work. Spectral analysis has been carried out for OM mantra to study its structure and to identify the factors, which have been found to influence the human nerve system. By this analysis, we could conclude stress gets minimized after OM chant.