Abstract: There are several reports that indicate a linkage between exposure to Extremely Low Frequency Electromagnetic Fields (ELFEMF) with a variety of biological effects. The aim of this study was to evaluate the influence of ELFEMF on endocrine secretion and structure of pancreas in rats. Experimental adult male wistar rats were exposed to a 50 Hz ELFEMF ,1 mT (emitted from solenoid) for 24 h daily during 135 days. The sham rats were subjected to sham exposure and the control rats were kept in animal room. In final, blood samples collected for the determination of the insulin, glucagon and glucose concentration in the plasma. The pancreas was examined using light microscopy. Results showed that in EMF exposed group insulin level was increased (p<0.01) which was associated with increased size of pancreatic islets and glucose level was decreased (p<0.01) but glucagon level did not significantly change. In conclusion, long term exposure to EMF impacts insulin secretion by influencing the structure of pancreas.