Abstract: The genetic manifestation of hybrid vigor among newly developed silkworm hybrid combinations over the parents was analyzed for the identification of superior cross breeds. Ten homozygous inbred polyvoltine breeds as Lines viz., APMG1, APMG2, APMG3, APMG4, APMW1, APMW2, APMW3, APMW4, APMW5 and APMW6 and three bivoltine breeds as Tester viz., APS8, APS12 and APS45 were used for the study. Adopting the LinexTester method, thirty hybrid combinations were prepared and reared at standard conditions. The data was measured on the nine important genetic traits viz., fecundity, yield per 10,000 larvae, pupation%, cocoon weight, shell weight, shell ratio%, filament length, reliability and neatness%. The data was analyzed for their Mid Parent Heterosis (MPH) and Better Parent Heterosis (BPH), six hybrid combinations viz., APMG1xAPS8, APMG1xAPS45, APMG3xAPS12, APMW1xAPS8, APMW2xAPS8 and APMW4xAPS45 were shown as significant heterotic combinations over mid parents for all the economical traits studied. The hybrid combination, APMW2xAPS45 with seven traits and APMG1xAPS8, APMG1xAPS12, APMG3xAPS12, APMW1xAPS8 and APMW1xAPS8 exhibited positive heterosis for six out of nine traits over better parent heterosis. Further, based on the evaluation index the study sturdily demonstrate that two new hybrid combinations viz., APMW1xAPS8 (59.58) and APMG1xAPS8 (58.68) were adjudicated as superior heterotic hybrid combinations and recommended for large scale laboratory trial.