Abstract: As a model animal for developmental biology, chick embryo is easy to control and observe during embryo development period and therefore it is widely used in the study of cardiac development. The application of proteomics has opened the door for large-scale studies to dissect both protein expression, regulation and function during chick heart developing stages. The proteomics study requires to quickly separate a large number of chick heart samples with the same developing stage. However, the traditional morphological standards based on Hamburger-Hamilton and Witschi stages are difficult to fulfill this requirement. Herein, we suppose a new standard for distinguishing chick heart morphology in different developing stages based on the relationship between the embryonic heart development and the amnion folding in chick. Based on this standard, we can accelerate the speed of embryonic heart sample separation and guarantee the quantity and quality of the sample more reliably.