Abstract: Background and Objective: High prevalence of viral infections and constant emergence of resistant virus strains worldwide calls for an urgent need to develop therapeutic agents to improve the treatment of virus infection especially against Herpes Simplex Virus-type 1. The objective of this study was to conduct phytochemical screenings of the methanolic/aqueous Hibiscus extracts and evaluate their antiviral activity as well as their therapeutic and prophylactic activity. Materials and Methods: Identification and evaluation of phytochemical ingredients and essential components of the Hibiscus extracts through different chemical analytical methods were conducted. Antiviral activity of Hibiscus extracts was evaluated using plaque reduction assay of the viral cytopathic effect as well as cell viability and proliferation assays using MTT, to evaluate the extract feasibility as a potential therapeutic agent. Experiments like viral binding and adsorption, virucidal and time of addition assays were designed to investigate the mechanism of antiviral activity of Hibiscus. Moreover, viral kinetics and antigen expression were demonstrated by fluorescence microscopy. MS Excel 2010 was used to provide statistical analysis like average and standard deviation. Results: Phytochemical analysis succeeded in extracting major constituents of Hibiscus like polyphenols, flavonoids and anthocyanin. Hibiscus showed a potential activity of methanolic and aqueous extracts against HSV-1 as indicated by selectivity index values of 8.0 and 7.7 correspondingly. Mechanism of action, revealed that the extract not only prevented the virus particles from interacting with the Vero cells in the pre-treatment assay but also had a prophylactic effect blocking the replication of the virus causing prophylactic selectivity indices of 6.1 and 5.2 for methanolic and aqueous extracts correspondingly, confirming a maximum protection of Vero cells against HSV-1 attack. Antigen expression Results showed that the effect of Hibiscus on inhibiting the HSV-1 virus after 5 h is much better than their effect 10 h post infection. Conclusion: Results demonstrated the potent and broad spectrum antiviral activity of Hibiscus due to the multiple components contained in the extract from active ingredients which cooperate together in synergetic manner and that all Hibiscus extracts tested were effective as virucidal and prophylactic agent, which makes Hibiscus a novel treatment against HSV-1.