Abstract: Developing DNA vaccines against avian viruses provides researchers to develop technology that offers new approaches for the prevention of emerging avian viral diseases. The DNA vaccine is ease to construction and manufacture and the potential for world-wide usage even in low-resource settings. The principle behind the DNA vaccine is injection of plasmid DNA encoding a foreign antigen of interest can result in the subsequent expression of the foreign antigen and the induction of an immune response within a host. This is relevant to prophylactic vaccination strategies that subsequently recognizes the infectious agent and fights off the disease. In this review we focused on the detail information on the development of vaccination strategies that include the incorporation of immunostimulatory sequences in the backbone of the plasmid, codon optimization, Kozak sequences, co-expression of stimulatory molecules, appropriate delivery methods for target antigens to increase the potency of the DNA vaccines, in understanding their immunological mechanisms that play a role to generate both cellular and humoral immune responses and in their applications and efficacy in clinical trials so far.