Abstract: The present study was Conducted determine the antibody response of an intradermal injection of trivalent inactivated influenza vaccine, with 40% of the usual dose and then to compare with the immunogenicity and safety of usual influenza vaccine, In this randomized control trial, we evaluated the antibody response of intradermal vaccine among 97 cases who were selected randomly from health care workers and then results compared with results in 94 cases of health care workers who were received intramuscular influenza vaccine with usual dose. One hundred ninety one cases with age range of 22-50 years, have been vaccinated by one experienced person then blood samples were evaluated for titers of hemagglutination- inhibition (HAI) antibody before and after injection of vaccine. Also, local and systemic adverse events assessed. Present study showed that there was no significant difference in seroconversion and seroprotection rates after vaccination, between two groups (p>0.05). Local reactions (induration and redness) were significantly more common, among recipients of intradermal injections than among recipients of intramuscular injection, but such reactions were mild and transient. We conclude that reduced dose of influenza vaccine given by the intradermal route in healthy adults was safe and immunogenic, similarly to intramuscular injection with usual dose and dose sparing with intradermal injection could be to increase the number of available doses of vaccine.