Abstract: Sugarcane mosaic potyvirus (SCMV) is the causal agent of mosaic disease which is reported to be one of the most important viral diseases attacking sugarcane crop in Egypt. Therefore, the present work was designed to screen the SCMV strains in Egypt based on biological, serological. and molecular characterization. Data of the use of Sorghum bicolor vars. Rio and Atlas show successful detection of 7 SCMV strains (A, B, D, E, H, 1, and M). The indirect-enzyme-linked immunosorbent assay (I-ELISA) detection of SCMV strains showed the presence of five strains, i.e., SCMV-A, SCMV-B, SCMV-D, SrMV-H and SrMV-I. The electron microscopy of SCMV-E-infected sorghum plant cells proved the presence of the cytoplasmic inclusions characterized to the potyviruses. The virus was purified and an antiserum containing polyclonal antibodies specific to SCMV-E strain was raised, Western blot immunoassay (WBIA) was Successfully used to study the specificity of the produced antiserum to SCMV-E strain. On the level of the molecular studies of SCMV-E strain, a single protein band with a size of about 35-37 KDa was obtained after SDS-PAGE analysis of the purified virus preparation. The SCMV-E-RNA was isolated from virus-infected sugarcane tissues and then used as a template for reverse transcription-polymerase chain reaction (RT-PCR) to amplify the c-DNA that directly used to amplify the coat protein (cp) gene of SCMV-E strain. The amplified cp gene was used as a template using the internal primer combination in PCR for confirmation its specificity to the SCMV-cp gene as a PCR product with a size of about 400 bp was amplified. The cp gene PCR product was cloned into the pGEM-T easy vector and introduced into Escherichia coli strain DH5α and the plasmid was then isolated, purified for sequencing the nested PCR product. The similarity between the present sequence (SCMVEgy1) and that of the 14 overseas strains belonging to different SCMV groups was determined.