Abstract: Laboratory incubation study gives insight to the condition of the field when treatment is applied. In order to determine the effect of cattle dung combined with urea fertilizer on soil pH and cations on soils from selected agro-ecological zones of Nigeria, an incubation study was carried out on four treatments in each of the agro-ecological zone. Soil samples were collected from Lagos (Mangrove forest), lpe Akoko (forest derived savanna), Lokoja (Southern Guinea savanna) and Katsina (Sudan savanna). Cattle dung was applied at the rate of 1.25 and 500 g soil to represent 5 t ha-1 and 0.05 and 500 g soil to represent 200 kg ha-1 urea fertilizer. Urea fertilizer was either applied singly or combined to give four (4) treatments in each of the agro ecological zone. The treatments were replicated three times and were arranged on Completely Randomized Design. Compared with control, cattle dung applied alone and cattle dung combined with urea fertilizer significantly increased (p<0.05) soil pH but at different level of increment in Lagos, Lokoja and Katsina while there were decreases in soil Al3++H+ from all the ecological zones. The treatments had no significant effect on Al3++H+ in the soil sample collected from Ipe Akoko. Soil types react to application of cattle dung and its combination with urea fertilizer in different ways in Nigeria.