Abstract: A field experiment was conducted in sandy clay loam soils to study the influence of sulphur on yield of sesame and nutrient uptake and available nutrient status at different growth stages of the crop during summer season of 2005 in Randomized Block Design (FRBD) with three replications. Three sesame varieties (TMV 4, TMV 6 and KS 95010) were tested with five levels of sulphur (S0: 0, S15: 15, S30: 30, S45: 45 and S60: 60 kg S ha-1). The available sulphur (S) was found to be higher at higher levels of S because of treatmental variation but the available nitrogen (N), phosphorus (P) and potassium (K) status in soil was decreasing with increased levels of S due to enhanced crop growth and development. The maximum S uptake was at 60 kg S ha-1 and N uptake was at 60 kg S ha-1 because increased S uptake accelerated increased N utilization. Maximum P uptake was with 45 kg S ha-1 due to the positive interaction i.e., S application might have been increased P availability in soil by reducing the soil pH. The K uptake was higher with 60 kg S ha-1 to KS 95010. Application 60 and 45 kg S ha-1 recorded higher seed yield and KS 95010 was significantly superior over varieties. The highest seed yield was obtained from KS 95010 with the application of 60 kg S ha-1. The physical optimal rate was 47.27 kg S ha-1 and the economic optimal rate was 46.09 kg S ha-1. The highest levels of S increased crop uptake and resulted in better seed yield. The results revealed that 60 kg S ha-1 increased the nutrient uptake and yield of sesame and maintain the soil available nutrient status and the optimum level of S can be fixed as 48 kg ha-1.