Abstract: Background and Background: Bovine blood is a livestock by-product that can be used as a protein source for livestock, particularly when incorporated in broiler rations. This study was conducted to investigate the effects of amino acids in Bacillus amyloliquefaciens-fermented mixtures of bovine blood and coconut pulp (blood meal) on growth performance, blood cholesterol and erythrocyte content in broilers. Methodology: A total of 100 six day-old CP 707 (Strain Cobb) broilers were divided into 5 experimental groups with 4 replicates of 5 broilers. The experimental groups received rations supplemented with 0, 5, 10, 15 and 20% blood meal that substituted for fish meal or soybean meal. The experimental period was 5 weeks. Treatment effects on broiler performance parameters such as final body weight, final weight gain, average daily gain, feed intake, average abdominal fat and Feed Conversion Ratio (FCR), total serum cholesterol and erythrocyte count, as well as the return on investment were determined. Results: The group fed rations supplemented with 10% blood meal showed final body weight, final weight gain and FCR of 1,172, 1,035 and 1.75 g, respectively, which was significantly higher (p<0.05) than that for the other treatments. Rations with 10% blood meal also had the best return on investment ($0.92) relative to the other groups. Conclusion: A mixture of 10% bovine blood and coconut pulp fermented with Bacillus amyloliquefaciens can be used in broiler feed without adversely affecting broiler performance and can replace 47% of total fishmeal and 53% of soybean meal in broiler rations.