Abstract: The aim of the present study was to determine the effect of dietary supplementation of fumaric acid on the performance, carcass characteristics and pH of different segments of gastro-intestinal tract (GIT) in broiler chicken. One hundred eighty, one week old broiler chicks were utilized for the study and distributed into four treatment groups, each having three replicates of 15 chicks each. Birds in the control (T1) group were fed the basal diet whereas in other treatment groups basal diet was supplemented with 0.5% (T2), 1% (T3) and 1.5% (T4) fumaric acid. Broiler chicken fed diets supplemented with fumaric acid showed a significantly (p<0.05) linear improvement in the body weight gains compared to control. Highest body weight gain of 1270.64±22.37 was observed in the group fed 1.5% fumaric acid in the diet (T4). There was a non-significant (p>0.05) effect on cumulative feed consumption in the birds among different treatment groups with lowest consumption in the control. Feed conversion ratio (FCR) improved in all the treatment groups fed fumaric acid in the diet with best FCR of 1.63±0.03 in T4. No significant (p<0.05) effect was observed in the carcass characteristics between various treatment groups including control. The pH of crop, proventriculus and gizzard decreased non-significantly (p>0.05), however no effect in the pH of different segments of small intestine was noticed. It may be concluded that fumaric acid proved to be a good substitute to antibiotic growth promoters in improving the performance of broiler chicken.