Abstract: The purpose of the present research was to evaluate the effect of dietary supplementation with different levels of zinc (0, 50, 75, 100 mg/kg diet) on semen quality of Cobb500 broiler breeder males. The four experimental diets were as follows: T1 = the basal diet (control) without any addition (0 Zn). T2 = 50 mg Zn (pure zinc)/kg diet. T3 = 75 mg Zn (pure zinc)/kg diet T4 = 100 mg Zn (pure zinc)/kg diet. The results showed that, Zinc supplementation had significantly (p<0.05) increased spermatocrit, ejaculate volume, mass motility, individual motility, while dead, abnormal sperm percentage and acrosome deformities were depressed (p<0.05) as affected by zinc supplementation throughout study. As well as, dietary treatments influenced sperm concentration and ejaculate volume during the experimental long. Moreover, results also revealed the superiority (p<0.05) of dietary treatments as compared with control group in respect of total mean of all semen quality.