Abstract: This experiment was conducted to evaluate the effect of different dietary levels (0, 5, 10, 15 or 20%) of Distillers Dried Grains with Solubles (DDGS). Each level was fed without or with two enzyme supplementation, (Kemzyme plus dry® supplemented at 250 gm/ton feed or Polytec Binder plus® supplemented at 500 gm/ton feed). A total number of 450 Inshas hens (30 wks old) and 45 cocks were distributed into 15 treatments of 30 hens, each in three replicates (10 hens and one cock, each). Diets were formulated to contain 16% CP and 2700 Kcal ME/Kg at laying period. Results show that no significant differences in digestibility coefficient values of Crude Protein (CP), Ether Extract (EE), Crude Fiber (CF), Nitrogen Free Extract (NFE), Body Weight Gain (BWG), feed intake and egg quality were observed by inclusion DDGS in laying hen diets. Also, results indicated that no significant effect on semen quality, fertility, hatchability and body weight of chicks in hatch by inclusion DDGS, enzyme supplementation or interaction between DDGS levels and enzyme supplementation in laying hen diets. Inclusion of 5% DDGS in laying diets significantly increased egg production %, egg number and egg mass, compared with the other levels. However, increasing DDGS to 15 or 20% in laying hen diets significantly increased yolk color and shell thickness and significantly decreased egg production %, egg number, egg weight and egg mass and gave the worst feed conversion compared with the other levels (0, 5 and 10%). Supplementation of Kemzyme plus dry® (enzyme 1) to diets containing DDGS had significantly higher percentage of digestibility coefficient value of ether extract and increased egg production %, egg number and egg mass than those fed the other treatments. In conclusion, the present results show that DDGS can be successfully fed at levels up to 10% in laying hen diet without adverse effect on laying performance. Also, enzyme supplementation could improve the utilization of DDGS to levels up to 20% of the diet.