Abstract: The influence of supplementing enzyme preparations made of three Aspergillus species (A. niger DB106, A. oryzae NM1 and A. niger XP) isolated from Vietnam on growth and performance of broiler chickens were investigated individually and in combination. In a completely randomized design, 270 of 30 day-old broiler chicks (Luong Phuong strain) were divided into 9 treatments with 3 replicate of 10 chicks per pen. Treatments were a dietary control (group 1) without enzymes and diets supplemented with enzyme preparations individually or in combinations to produce the other treatments (group 2-9). At the end of the feeding trial, 6 chicks per replicate were sacrificed to evaluate carcass and meat characteristics. The results as experiment finished of 5 weeks later demonstrated that the body weight gains were improved in approximately 6-16% with enzyme addition individually and from 14.5-27.2% (P < 0.05) with addition of enzyme combinations. The effect of the combination of enzyme preparations from three species of Aspergillus showed the highest efficacy on BWG of chickens (27.2% compared to control group), which were much higher than the effect of Green enzyme (11.6%) from China that is available in Vietnam market. Feed conversion ratio (FCR) was reduced in all experimental groups compared to the control (P < 0.05). The meat quality and carcass characteristics of chickens in all groups were not altered under the effect of the enzyme preparations. This finding demonstrated that these enzyme preparations are safe and very prospective preparation for the fattening broiler chickens.