Abstract: Chicken Infectious Anemia Virus (CIAV) is a resistant and ubiquitous virus causing disease in young chickens and immunosuppression in all birds. CIAV infection in broiler chicken flocks has been described in most countries with a developed chicken industry and can result in economically important losses either clinical or subclinical form of the disease in broiler chickens. In this study 414 sera samples from 32 commercial broiler chickens flocks located in Northern Jordan, were tested for the presence of CIAV antibodies using a commercial enzyme linked immunosorbent assay kit. Overall seroprevalence in broiler chicken populations was 82.61%. The rate of antibody-positive chickens among flock`s samples ranged from 38.5% to 100%. The seropositive samples were more obvious in older chickens. This is the first report of serologic evidence of CIAV in Northern Jordan. According to these results, the disease can probably be found throughout the country and beyond. Therefore further studies are necessary to assess economic losses due to CIAV and the cost benefit of countermeasures.