Abstract: The nutritive value of cereal as a major ingredient of poultry feeding may be affected by environmental condition. This reflection may influence poultry production particularly egg production. One hundred and eighty Leghorn pullets were placed in individual cages. They were divided into 9 treatments, four in 20 and 40% of Alvand wheat and Makoie barley (as a cold cereal area). Next four in Chamran wheat and Karoun barley with the same percentage (as a tropical cereal area) were used with control diet. Chemical composition was similar in examined cereal with exception in barley fiber which was higher than others. There were no significant differences in body weight (BW), feed intake (FI) and feed conversation ratio (FCR) between cold and tropical cereal area as well as control diet. Egg weight and eggshell thickness were significantly higher (P<0.05) in the control diet compared with the other treatments. The similar trend was appeared in the yolk color (P<0.05). Haugh units were significantly greater (P<0.05) in 20% of Karoun barley as well as in 20% of Alvand wheat compared with other reflections. On the other hand no significant differences were observed in the egg production, egg shell weight, albumin height and specific gravity in treatments. The result of this study has shown that overall Leghorn performance had similar reaction in response to tropic and cold cereal area with exception in 40% of Karoun barely, which reduced this potential.