Abstract: Infectious bursal disease is one of the most important viral disease of poultry usually affects young chickens of 3-6 weeks. Hygienic management and proper vaccination are main way of control of this disease. But maternal antibody affects vaccination with live vaccine. To determine the effect of maternally derived antibody on live vaccine, the study was conducted. A total of 100 day old chicks (50 from vaccinated parent stock and 50 from non-vaccinated parent stock) were used in this study. A preset vaccination schedule was followed for chicken and blood samples were collected to find out the actual effect. It is observed that day old chicks contain high level (6294.14±24.95) of maternally derived antibody which gradually decline below positive level within 15-20 days (390.45 ±19.42) and half-life is about 5 days. Vaccination of chicken with high level of maternally derived antibody interferes with the vaccine virus results no immune response but revaccination provokes immune response. Better immune response is found in chickens vaccinated at day 21 and boostered at day 28. But there may be chance of infection because maternal antibody declined below positive level within 15-20 days. Chickens from non-vaccinated parent stock shows good immune response from first time that is from primary vaccination at day 7 and boosting at day 14.