Abstract: Bacterial leaf blight is a major disease in all rice countries and a constraint in hybrid rice production. This study was undertaken to insulate the most popular parental lines of hybrid rice KMR3 and PRR78 (restorer lines) and IR58025B and Pusa 6B (maintainer lines) against the disease by pyramiding bacterial blight resistant genes into each background. Molecular markers are vital components in breeding programs involving gene pyramiding. Using marker-assisted selection in a-breeding program, four bacterial leaf blight resistant genes namely Xa4, xa5, xa13 and Xa21 have been introgressed into the restorer and maintainer lines. Foreground selection was done using markers. Conventional breeding strategy was adopted for background selection. The pyramids showed very high level of disease resistance to 10 highly virulent isolates of Xoo. Grain quality parameters of the pyramids were on par with that of the original genotype. This work demonstrates the successful application of marker-assisted breeding for pyramiding four BB resistant genes into multiple backgrounds, simultaneously. These lines can be used directly or as donors of Bacterial Blight (BB) resistance breeding.