Abstract: Background and Objective: Locally adapted varieties are the genetic resources used to develop bettered varieties. Chinekani, a local rice variety with a pleasant aroma and great quality is cultivated in the salty region of Bangladesh. However, no improved rice variety or mutant was included in a mutant database of FAO from this local rice variety. Therefore, the main objective of this study is to evaluate the effects of Ethyl Methane Sulfonate (EMS) on creating a mutant population of this local rice variety. Materials and Methods: To evaluate the effect of ethyl methanesulfonate on genes of local rice relating to the morpho-physiological and yield-related character, different concentrations of EMS (1, 2, 3, 4 and 5%) for a three-time duration (6, 12 and 24 hrs) was applied and its effect was observed till harvesting. Results: In this study, LD50 was observed at 3% concentration for 6 hrs based on germination rate indicated higher concentration of EMS requirement for successful mutation breeding. Moreover, total chlorophyll (a+b) content remained unchanged and carotenoid content was significantly decreased in all treatments compared with control pointed to the non-mutagenic effects of EMS on genes responsible for chlorophyll as well slowing down effect on carotenoid related gene(s). In M2 generation, significantly increased leaf area, meaningful tiller number per panicle and a significant number of grains per panicle were found among the mutants. Conclusion: Findings of this investigation can be exploited for future rice breeding programs. Novel mutant alleles and their functional gene expression analysis employing reverse genetic tools might be discovered using these germplasms.