Abstract: Background: Cotton is the most important fiber crop and understanding the genetic mechanisms controlling fiber production on cotton seeds can aid in the development of improved varieties with higher lint yields and improved fiber quality. Lint and fuzz are the two types of fiber produced on the cotton seed and mutations in the genes controlling these traits can result in a lack of fiber development. The ovule fuzzless genotype PI 529708 was identified from the United States Gossypium arboreum germplasm collection. Methodology: This genotype was crossed with the fuzzy seed genotype PI 529729 to determine the genetic inheritance of the ovule fuzzless trait. Results: Seeds from F1 plants showed the ovule fuzzless phenotype indicating the fuzzless trait was dominate over fuzz fiber development. The F2 segregation data fit a three complementary gene model. For corolla color, petal spot and stem pigmentation, the F2 segregation data for each trait fit the expected single dominant gene model indicating normal segregation for the population. Conclusion: Reducing the amount of fuzz fibers on the cotton seed is one strategy to improve lint yield and ginning efficiency, therefore, determining the genes controlling fuzz fiber production is required to manipulate this trait in breeding programs.