Abstract: To evaluate the genetic diversity among the 33 sorghum accessions of Sudan, 70 Simple Sequence Repeats (SSR) and 23 Sequence Related Amplified Polymorphism (SRAP) primer sets were utilized. Results indicated that, of the SSR markers used, 50 (71.4%) were polymorphic, producing 88 (53.0%) polymorphic alleles of PIC value ranging from 0.06 to 0.96 with an average of 0.58. Among the SRAP markers, 8 (34.8%) produced 33 alleles with 48.5% marker polymorphism and PIC value ranging from 0.23 to 0.93. The similarity coefficients based on SSRs were in the range of 0.15 to 0.78 with an average of 0.45, while those based on SRAP markers were in the range of 0.13 to 0.80 with an average of 0.50. Coefficients of similarity generated with SSRs grouped the sorghum accessions into five clusters. In contrast, grouping according to the similarity coefficients of the SRAPs resulted in another completely different five clusters. When data obtained from both SSRs and SRAPs were combined and utilized to generate a dendrogram in which the 33 sorghum accessions were again grouped into five clusters. Cluster 1 and 5 were typical to those of the SSR-based grouping. The present study indicates that fast, accurate and high throughput fingerprinting could be obtained using those markers, from the combined analysis, which revealed the existence of significant variation among the 33 accessions. The most distant accessions can be used by breeders to develop improved sorghum genotypes.