Abstract: The aim of this study was to identify and fingerprint the mutants of sunflower plants using RAPD analysis. Ten mutants of two cultivars Giza 1 and Giza 102 of Helianthus annuus produced by sodium azide treatments and the control were evaluated by RAPD markers. The mutants of cultivar Giza 102 were as follows: mutant 1 which changes in the form of inflorescence, mutant 2 is a dwarfed plant, mutant 3 is a plant with large leaves, mutant 4 is a plant with many inflorescences, mutant 5 is a dwarfed plant also. The mutants of cultivar Giza 1 were as follows: mutant 6 with reddish leaf petiole, mutant 7 is a dwarfed plant, mutant 8 with changes in the leaf form, mutant 9 is a plant with dark green inflorescence calyx, mutant 10 is a plant with stripped ray florets. Ten random primers were used; eight out of ten primers used showed variations among the mutants. The 98 amplified fragments were yielded, 83 bands were polymorphic and the total polymorphic rate was 84.6%. Some fragments were common for both genotypes and others were absent as a result of treatments. A dendrogram based on the genetic distance grouped the mutants and the two control cultivars to three clusters A, B and C. The polymorphism detected in all the mutants with both genotypes suggested that sodium azide could be used as effective mutagenic agents in sunflower. RAPD marker is a powerful technique enables fingerprinting of selected mutants in breeding of sunflower plants.