Abstract: An attempt was made to analyze the variability and correlation of characters among five accessions of Tribulus terrestris collected from different geographical and agroclimatic regions of South India. All the five accessions showed different levels of morphological and phytochemical variability. Leaf area showed the maximum morphological variability. Phytochemical analysis by using HPLC revealed differential levels of chemical affinities. Among the six morphometric characters studied, all the characters except internodal length showed significant positive correlation towards each other. Results from this study can be exploited for commercial and breeding purposes of this important medicinal plant.