Abstract: Background and Objective: Osteoarthritis (OA) is the most complex joint condition in the world. It affects many joints in the body and is a leading cause of disability. The objective of this study was to evaluate the efficiency of Dipsacus asperoides (DA) extract in improving physical performance and regulating oxidative stress in an animal model of monosodium iodoacetate-induced Osteoarthritis (OA). Materials and Methods: To study the physical performance, rats were tested for the wire hang test, von frey test, open field test, rotarod test and hotplate assay were tested. After the examination period, the drug-administered animal's tissue homogenate was analysed for the biochemical estimations. Results: In this investigation, group VI exhibited the longest PWT time duration in the DA-administered groups in the von frey assay. Group VI achieved the longest hanging time (68 sec) in the hang test. Group VI exhibited a minimum of 51 movements in 5 min in an open-field test. In the rotarod experiment, group VI animals took a longer time (168 sec) to fall off the rotarod. Groups V and VI as well as group II, had a strong withdrawal response to heat stimulation in a hotplate test. In the biochemical analysis, DA administrated animals exhibited significant outputs compared to the other tested animals. Conclusion: The present study demonstrated that the phytochemical components that engage in physical performance improve osteoprotection and cure OA-related diseases.