Abstract: Background and Objective: Allergic Rhinitis (AR), is a nasal mucosal inflammation that arises due to overreacts by the immune system to inhaled allergens and is a common and serious public health problem and affected a large number of the world population. The current experimental study scrutinizes the antiallergic effect of hesperidin against the Ovalbumin (OVA) induced Allergic Rhinitis (AR) in mice via anti-inflammatory and antioxidant parameters. Materials and Methods: Wistar mice were divided into six groups, non-sensitized, ovalbumin sensitized, sensitized treated with hesperidin (5, 10 and 20 mg kg–1) and montelukast (10 mg kg–1) for 21 days. On the day of 21, intranasal OVA was induced and end of the experimental protocol spleen weight, biochemical, physiological, Interleukin 4 (IL-4), Interleukin 6 (IL-6), Interleukin 10 (IL-10), Immunoglobulin-E (IgE), Interferon-gamma (IFN-γ), IFN-γ/IL-4 ratio, total protein, phospholipase A2 (PLA2) were estimated. However, the mRNA expression of GATA-3, T-bet, FOXP-3 and (ROR)-γt were also estimated. Result: Hesperidin significantly (p<0.001) showed the protection against sneezing, nose redness and nasal rubbing followed nasal challenge. Hesperidin considerably (p<0.001) down-regulated the histamine, nitric oxide production, IL-4, IL-6, IL-10, IgE, TP and PLA2 as compared to the OVA-induced RA. Hesperidin considerably reduces the ratio of IFN-γ/IL-4 as compared to the OVA-induced RA. Hesperidin considerably down-regulated the mRNA expression of ROR-γt, GATA-3 and up-regulation of FOXP-3 and T-bet expression at concentration-dependent manner. Conclusion: Based on the result, we can conclude that hesperidin may ameliorate the OVA-induced AR condition via alteration of expression of ROR-γt, GATA-3, FOXP-3 and T-bet, which commit T helper cells to TH1 phenotype.