Abstract: Background and Objective: The gastric ulcer arising from many reasons is an important disease and is developed too often by the excessive consumption of alcohol. In this study, the potential gastric protection effect of the magnolin having significant pharmacological effects against the acute gastric damage induced by the ethanol/HCl in mice is examined. Materials and Methods: The study was carried out with a total of 49 Swiss albino mice, control, dimethylsulfoxide, omeprazole, ethanol/HCl and in addition on the groups given 3 different doses of magnolin (2.5, 5 and 10 mg kg–1). The effect of magnolin was examined on gastric damage with the macroscopic, biochemical and proinflammatory cytokines mRNA expression analysis of the gastric tissue. Results: As a result of the study, it was observed that orally administered magnolin was significantly effective in alleviating macroscopic and histopathological lesions caused by ethanol in the gastric mucosa, improved antioxidant activity, prostaglandin E2 and nitric oxide levels and decreased lipid peroxidation in the gastric mucosa. However, it was also found that pro-inflammatory cytokine expressions induced by ethanol were decreased by the application of magnolin. Conclusion: The findings of the study showed that the magnolin represses the lipid peroxidation and inflammatory response, on the other hand, can exhibit a protective effect against in vivo ethanol-derived gastric tissue by increasing activation of the antioxidant system.