Abstract: Background and Objective: Non-alcoholic Fatty Liver Disease (NAFLD) is a disease of excessive lipid accumulation which sensitizes the liver cells and leads to hepatic insulin resistance, inflammation and oxidative stress. This study was aimed to evaluate the hepato-protective effect of Byrsonima sericea extract (BSE) against High fat emulsion diet (HFED) induced NAFLD in rats. Materials and Methods: The NAFLD was induced by administration of HFED (10 mL kg–1/p.o.), along with micro dose of carbon tetra chloride (CCl4) (0.5 mL kg–1, i.p.). The experiment includes 5 groups with 6 rats in each group. Group 1 was normal control, Group 2 was negative control, where rats received HFED (10 m kg–1 p.o, B.W) and CCl4 (0.5 mL kg–1, i.p.). Group 3, 4 and 5 were BSE treated rats in a dose of 75, 150 and 300 mg kg–1, respectively. All rats were tested for physical as well as biochemical estimations like ALP, blood glucose, insulin and bilirubin content. Histopathological examination of liver was also performed in trial groups of rats. Results: The extract at a dose of 300 mg kg–1 (i.p.) showed significant protective effect by reducing the higher content of cholesterol, triglycerides, LDL and VLDL level in serum of rats. It also showed a potential decrease in ALP, total bilirubin and SGOT content in serum. The rats treated with extract showed less hepatic damage in liver cells as compare with negative control group rats. Conclusion: This study concluded that the rats treated with BSE have significant hepatoprotective action and acts by modulating the hepatic enzymes secretion, via reducing weight gain and organ fat deposition in rats.