Abstract: Wnt ligands play a crucial role in development, tissue turnover and human diseases. The extracellular matrix protein Wnt7b, a critical regulator of hundreds of genes is involved in the development, invasion and metastasis of tumor. Universally, high expression of Wnt7b occurs in tumor lesion compared with normal tissue and is related to clinicopathological characteristics and poor prognosis. Recently, the new mechanism of “canonical or non-canonical” Wnt7b signal pathway has been revealed at cellular and molecular level. The molecular function of new receptors of Wnt7b in the membrane has been characterized and the relationship between Wnt7b/β-catenin classical pathway and transcription factor including TGF-β, VEGF, MicroRNAs and related factors of EMT has been increasingly clear. Moreover, “non-canonical” pathway of Wnt7b rather than activating β-catenin plays a critical part in invasion and metastasis of tumor through PKC and stat3. Here, the available research for the role of Wnt7b on invasion and metastasis tumor was reviewed.