Abstract: Background and Objective: Ethyl acetate fraction of Myrmecodia tuberosa Jack. increases phagocyte activity of macrophage and lymphocyte proliferation and also prevents cutaneous anaphylactic reactions. Based on that, this present research aim to investigate the effect of an acetate fraction from Myrmecodia tuberosa Jack. on numbers of erythrocyte, reticulocyte, hemoglobin content and hematocrit in mice. Materials and Methods: The research was conducted over 3 months and consisted of a positive control group and 3 groups treated with Myrmecodia tuberosa Jack. ethyl acetate fractions at 3 dosing levels. Anemia was induced in the mice using chloramphenicol 130 mg kg–1 b.wt., for 14 days then for next 14 days daily oral doses of 40 mg kg–1 b.wt., 63.2 mg kg–1 b.wt., or 100 mg kg–1 b.wt., of Myrmecodia tuberosa Jack. ethyl acetate fraction were administered to each group. Blood samples were taken on day 0, 14, 21 and 28 for analysis. Statistical analysis was conducted using two-way ANOVA then Duncan Multiple Range Test (DMRT). Results: About 40-63.2 mg kg–1 b.wt. doses of Myrmecodia tuberosa Jack. ethyl acetate fraction significantly increased the erythrocyte, reticulocyte and hemoglobin count and hematocrit from the 14th day (p<0.01). Conclusion: Ethyl acetate fraction of Myrmecodia tuberosa Jack. could have potential as an anemia treatment.