Abstract: Background and Objective: Potassium dichromate (K2Cr2O7) is an environmental contaminant widely recognized as a carcinogen, mutagen and teratogen toward humans and animals. The objective was to study the toxicity induce by K2Cr2O7 and to evaluate the possible ameliorating effect of vitamin-C on potassium dichromate (K2Cr2O7) induced oxidative stress and generation of inflammatory response in rats. Materials and Methods: Adult male wistar rats were randomly divided into 4 groups of five animals each: Group I-N received single i.p., injection of normal saline and served as normal control group; group II (K2Cr2O7)-K group was subjected to a single i.p., injection of potassium dichromate (15 mg kg–1) to induce toxicity; while the group III (K2Cr2O7+Vitamin C)-CK group was pretreated with single i.p., injection of vitamin-C (250 mg kg–1), 6 h prior to administration of potassium dichromate. The group IV (Vitamin-C only)-C group received single i.p., injection of vitamin-C in saline (250 mg kg–1). The body weight of each animal was recorded before and after completion of the respective treatment. Oxidative stress markers like MDA, glutathione levels and serum interleukins (IL-10, IL-1α, IL-6, IL-8, IL-18 and TNF-α) were determined in all of rats studied. Comparison between the groups was performed by one way ANOVA followed by Holm-Sidak test. Pearson’s correlation coefficient was performed to study the correlation between interleukins in K and CK group. Results: The K2Cr2O7 administration increased serum IL-6, IL-8, IL-10, IL-18 and TNF-α levels significantly (p<0.001) compared to saline treated control group. The levels of MDA and glutathione were altered significantly (p<0.001) in dichromate treated group compared to the control. These changes were reversed significantly (p<0.001) in animals receiving a pretreatment of vitamin-C. Conclusion: It is concluded that potassium dichromate is known to induce oxidative stress and inflammation in rats and the toxicity is reversed by the chemoprotective and anti-inflammatory property of vitamin-C.