Abstract: Background: Sarenta a decoction from medicinal plants was prepared by a traditional health practitioner. However, there is no scientific data that can back up its reliability for consumers. The main objective of the study was to evaluate the know-how of Ivoirian’s traditional health practitioners. Objective: The aim of this study was to carry out an ethnopharmacological survey about Sarenta, evaluate its analgesic activity, its quality through research of microbial contamination and eventually assess its acute toxicity. Materials and Methods: The ethnopharmacological survey was performed using an open questionnaire. The analgesic activity assessment through behavioral tests, abdominal constriction test in mice and formaldehyde-induced paw oedema test in rats. Thus, the microbial enumeration test based on the French pharmacopoeia and toxicity test 423 of Organization for Economic Cooperation and Development, helped to respectively evaluate microbial contamination and acute toxicity. Statistical test was performed using Wilcoxon test to compare groups. Results: The recommended dose set up by the traditional health practitioner was about 3 mg kg–1 b.wt. for adults. “Sarenta” at a dose of 5.10–8 mg kg–1 b.wt. (a preparation of 50 mg mL–1 was diluted to 10 millionth leading to 5.10–7 mg mL–1, which was administered to rats at 1 mL/100 g b.wt.) inhibited abdominal constrictions in mice by 76% and licking of injected paw in rat by 70% at the inflammatory phase. Therefore, no fecal contamination germ, no yeast and mold were found beyond the French pharmacopoeia standards. Moreover, no deaf of rat was recorded at a dose of 5000 mg kg–1. Conclusion: “Sarenta” exhibited analgesic and potential anti-inflammatory properties, a microbiological quality that complied with the French pharmacopoeia standards and contains no acute toxic substance. Thus, this first scientific study on herbal preparation could promote the know-how and professional practice of Ivoirian’s traditional health practitioners.