Abstract: Background and Objective: The present study was aimed to investigate the effect of polyherbal formulation Rumalaya Forte on experimentally induced insulin resistant myocardial infarction in rats. Materials and Methods: The effect of polyherbal formulation Rumalaya Forte (160 and 320 mg kg–1 b.wt., p.o.) was studied on the high fat diet induced insulin resistant myocardial infarction animal model. Vitamin E (100 mg kg–1 b.wt., p.o.) was used as standard. The study was evaluated with the help of various biochemical parameters and histopathological examination. Results: Results showed that the Rumalaya Forte treatment prevented myocardial infarction, increase in blood glucose level, body weight and weight gain due to antioxidant property and showed a significant dose dependent decrease in the parameters like alanine aminotransferase, aspartate aminotransferase, creatinine phosphokinase, total cholesterol, total triglycerides, weight and number of adipocytes and in infarction size. The histopathological examination also reveals the significant protection in the Rumalaya Forte treated group with a minimal or no red coloration in a dose dependent manner. Rumalaya Forte 160 mg kg–1 b.wt., p.o. groups showed relatively less disruption of myofibers with Rumalaya Forte 320 mg kg–1 b.wt., p.o. and vitamin E showing maximum fiber integrity. Rumalaya Forte 320 mg kg–1 b.wt., p.o. was found to be more effective compared to Rumalaya Forte 160 mg kg–1 b.wt., p.o. Conclusion: The pleiotropic effect of Rumalaya Forte can be utilized as add on therapy for treatment of myocardial infarction.