Abstract: Background: Tetracaine hydrochloride is a topical local anaesthetics used in the treatment of pain associated with minor surgical procedures it also gives relief from pain associated with various localized muscle, joint, post-herpetic neuralgia, arthritis and haemorrhoids. Objective: The present study is designed to prepare and evaluate transdermal patches of tetracaine hydrochloride to deliver drug for a longer period of time to circumvent severe pain after surgical procedures. Methodology: Transdermal patches of tetracaine hydrochloride were prepared by solvent casting method. The prepared patches were evaluated for the various evaluation parameters like thickness, surface pH, weight uniformity, content uniformity, folding endurance, swelling index, in vitro drug release study, in vitro test for mucoadhesion and in vivo studies. Results: All the formulations exhibited acceptable physical properties. In ex vivo drug diffusion study the patches exhibited controlled release upto 24 h. The formulation Th3 (containing EC, SCMC and PVP) showed the best performance in the ex vivo drug diffusion with 92.02±1.21% drug release after 24 h. In the clinical study on the patients suffering from the post operative pain it was observed that the Th3 decreased the pain score from 9.89±0.08 to 4.05±0.21 while the gel could decrease the same from 9.80±0.01 to 7.09±1.10 at the end of 24 h post application. Conclusion: It was concluded that the prepared transdermal patches of the tetracaine were having significant effect as compared to the tetracaine gel used conventionally.