Abstract: Background: The occurrence of Disseminated Intravascular Coagulation (DIC) could lead to multiple organ failure, promoting the development of the critically ill patient’s condition. Recently pharmacological studies had shown that andrographolide had effects on anti-inflammatory and preventing thrombosis. Materials and Methods: In order to monitor, if andrographolide has protective effects on DIC rabbits, the DIC models were established by infusing lipopolysaccharide (LPS) in 60 mL of saline solution at a rate of 10 mL/kg/h through the rabbits’ marginal ear vein over a period of 6 h. The DIC rabbits were divided into 5 groups; low (1.0 mg kg–1), medium (2.0 mg kg–1), high (5.0 mg kg–1) andrographolide groups, LPS (saline solution) group, heparin (500 IU kg–1) group. The normal control group treated with saline solution, wI-1 and t-PA in seruhich was given neither LPS nor andrographolide before. Survival rate was monitored by recording the deaths within 6 and 24 h after drug treatment and renal function was detected, including Blood Urine Nitrogen (BUN) and tissue section. Moreover, the concentrations of TNF-α and IL-1β were determined and the activities of ATIII, protein C, PAm level were measured, which was collected at 2 and 6 h post-infusion. All data was presented as Mean±Standard Deviation. Survival rate was analyzed by chi-square test. Differences between two groups were analyzed by one way ANOVA analysis. Results: Treatment of andrographolide significantly increased survival rate and reduced renal damage and increased the activities of protein C, the levels of ATIII and t-PA. But the levels of FDP and PAI-1 were decreased and the suppression of TNF-α and IL-1β secretion could be found as well. Conclusion: All the data suggested that had positive effects in intervention in DIC rabbits and prevented excessive inflammation via inhibiting the level of TNF-α and IL-1β in serum. Andrographolide could be a potential drug for DIC treatment.