Abstract: Oxidative stress has been suggested to explain the mechanism of numerous diseases and antioxidants have been proposed to prevent cellular oxidative damage, therefore disease progression. Antioxidant capacity of many plants, fruits and vegetables have been studied by the researches, however there was no data regarding the antioxidant properties of Gypsophila bitlisensis. The species is an endemic and natural plant of Turkey. We performed three assays to reveal antioxidant capacity of ethanol extract of Gypsophila bitlisensis: FRAP, DPPH and CUPRAC. Trolox and α-tocopherol have been used as standards. We also determined phenolic content of the extract. The results showed that Gypsophila bitlisensis does not have significant antioxidant capacity and phenolic content. On the basis of these findings, we concluded that Gypsophila does not have considerable antioxidant activity in these in vitro assays.