Abstract: Essential oils, volatile compounds from plants, are attractive natural ingredients possessing wide range of applications in cosmetics, foods, household products and alternative medicines. In this study, the three different oils from Prunus yedoensis (PYE), Saururus chinensis (SCE), Zanthoxylum piperitum (ZPE) were prepared by supercritical carbon dioxide extraction and their chemical components were analyzed. In addition, their anti-inflammatory activities were investigated by measuring the inhibition of pro-inflammatory cytokines. Monoterpenes α-Pinene (22.4%), 2-β-pinene (20.1%) and camphor (13.1%) were found as the major components in PYE. In the case of SCE, sesquiterpenes δ-cadinol (22.5%) and δ-cadinene (19.7%) as well as a diterpene trans-phytol (13.7%) were mainly identified. The major chemical constituents of ZYE were in the order of octanoic acid (13.4%), n-heptanol (9.8%) and 1-octanol (8.1%). As a bioactivity study, the effects of PYE, SCE and ZPE on nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 production in lipopolysaccharide-activated RAW 264.7 macrophages were examined. These essential oils (PYE, SCE, ZPE) were appeared to considerably suppress the production of pro-inflammatory cytokine and mediators in dose-dependent manner. The results indicate that PYE, SCE and ZPE can be useful natural agents to manage inflammatory symptoms and diseases.