Abstract: Bee Venom (BV) has been used as treatment against a variety of inflammatory diseases. Studies have demonstrated anti-inflammatory effect of BV. This study aimed to clarify the potential clinical efficacy of BV concerning the anti-inflammatory effect on bronchial epithelial cell. BEAS-2B cells were exposed to Alternaria alternata and Aspergillus nigra with or without BV. IL-6, IL-8 and granulocyte macrophage colony-stimulating factor were measured to determine the activation of epithelial cells. Nuclear factor kappa B (NF-κB) and activator protein 1 expression and activity were determined with Western blot analysis and ELISA. Cytotoxicity of BV was measured using a CellTiter-96® aqueous cell proliferation assay kit. Cell survival was significantly decreased concentrations at 10 μg mL-1. Fungi induced IL-6 and IL-8 production was effectively inhibited by BV. BV suppressed fungi induced NF-κB expression and Alternaria induced NF-κB activation. BV seems relatively safe and is of potential value for the treatment of airway inflammatory diseases.