Abstract: The kidneys are important organs in the process of filtering blood. Damage of the renal function often leads to Chronic Kidney Disease (CKD). Anredera cordifolia leaves, traditionally, are used to treat various diseases, including kidney failure. Therefore, this study aimed to determine the effect of three different doses Anredera cordifolia leaves extract against renal function improvement in renal failure conditions. The research includes quality analysis and testing of the effects of Anredera cordifolia leaves extract to kidney failure. Rat model of renal failure were developed using gentamicin (100 mg kg-1 b.wt.) intraperitoneally and piroxicam (3.6 mg kg-1 b.wt.) orally for 8 days. Anredera cordifolia extract (50, 100 and 150 mg kg-1 b.wt.) was given from day 8 for four weeks. Evaluation of renal function included serum creatinine levels, serum urea levels, organ index and kidney’s histology. After four week of therapy, Anredera cordifolia extract at doses of 50, 100 and 150 mg kg-1 b.wt. decreased creatinine levels (0.02±0.17, 0.07±0.13 and 0.05±0.12) mg dL-1 which were significantly different to the positive control group. Urea level decrease also occurred significantly in the test dose of 150 mg kg-1 b.wt. of 20.35±2.89 mg dL-1. Anredera cordifolia extract dose of 150 mg kg-1 b.wt. significantly influenced on renal index. Histological results in the three test group also showed improvement in renal cells after administration of the extract. Extracts on all three types of test dose provided improvements to the kidney function.