Abstract: A simple and sensitive assay method was developed for simultaneous determination of cefepime and sulbactam sodium in Supime (a fixed dose combined formulation of cefepime and sulbactam manufactured by Venus Remedies limited, India) with UV detection at 230 nm. Chromatographic separation of two drugs was achieved on a Hypersil ODS C-18 column using a binary mixture of acetonitrile and tetrabutyl ammonium hydroxide as a mobile phase adjusted to pH 5.0 with orthophosphoric acid in ratio 20:80 v/v ratio. The developed liquid chromatographic method offers good linearity, accuracy and precision over the concentration range of 125-750 ppm for cefepime and 62.5-375 ppm for sulbactam sodium. This method was successfully applied for the quality control of formulated products and plasma samples containing Cefepime and sulbactam. Since, Supime, a fixed dose combination of cefepime and sulbactam is a research product of Venus Remedies limited the literature lacks any method of analysis for such combination, the main motive behind this experiment was to develop and validate a method which could be used for the quality control of cefepime and sulbactam in combined dosage form.