Abstract: The fresh juice obtained from the locally grown radish root was tested for possible hepatoprotective effect against carbon tetrachloride-induced hepatocellular damage in albino rats. The juice at two doses of 2 and 4 mL/kg/rat for five consecutive days, exhibited a significant dose-dependent protective effect. The magnitude of protection was measured by using biochemical parameters including determination of Serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), alkaline phosphatase (ALP), bilirubin (Bil) and non-protein sulfhydryl content in the liver tissues (NP-SH), in addition to histopathological assessment. The protective effect was demonstrated in lowering the elevated serum levels of SGPT, SGOT, Bil, ALP and increasing NP-SH level. Silymarin, a known hepatoprotective agent was used as a positive control. Biochemical data were further supported by the histopathological results. The phytochemical examination of the fresh juice revealed the presence of sulfurated, phenolic and terpenoid compounds in radish.